The researcher has no limitations on access to the data, except that she requires that those using or referencing her data cite the paper from which it came rather than the data itself. This data is publicly available once the article is published. The researcher submits some of her data to a database or repository as a part of the publication process. It is also very important for her students to be trained to properly document their experiments using her lab notebook structures and protocols. Therefore, keeping an organized electronic lab notebook is of high importance for the researcher. The lab notebook contains written documentation of all experiments and trials and links to data, versions of analysis and images, databases, and versions of the developing journal article. Cells on porous PCL also displayed significant upregulation of key RPE proteins including a 6.45-fold increase in RPE65 and 2.19-fold increase in CRALBP. The stages of her research involve a long period of methodology development, a shorter data collection period, and then data analysis.
She will do transcriptome and high throughput ChIP-seq analysis on the cell nuclei to examine the distribution of complexes that modify histones and how loss of those complexes affects transcription. Statement of significance: Mesenchymal stem cells (MSCs) have great therapeutic potential, as they are capable of multilineage differentiation. Specifically, the goal is to sort nuclei from glial and neuronal cells from the brains of mutant and wild type drosophila larvae. Taken together, these results suggest that 3D printed scaffolds with ordered cubic pores could be a suitable culture system for single-step MSC enrichment and differentiation. The researcher is conducting research on the brain cells of drosophila larvae.
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